Biotechnology

Steve Scheidell 
Last updated on  May 10, 2013

Month

Content

Learning Activity

Core Standard

August/ September

  1. History of Biotech
  2. Lab Safety & Lab write-ups
  3. Chemistry 
  4. Lab Equipment
  5. Conversions
  6. Solutions/Dilutions
  7. Spectrophotometry
  1. Pipetting 
  2. Solution Preparation
  3. Serial Dilutions 
  4. Spectrophotometric Analysis
  1. 1.01    History of Biotech
  2. 1.03 & 1.04   Biotech Careers
  3. 2.01 - .04 Safety
  4. 01.01 - 01.03 Chemistry 

October

  1. Biochemistry
  2. Protein structure & function
  3. DNA structure & function
  1. Protein purification Chromatography 
  2. Protein Analysis SDS Page
  3. Enzyme activity assay
  1. 02.01 - 02.03  Biochemistry

November

  1. DNA replication - protein synthesis
  2. Genetic code
  3. DNA technology
  4. Restriction Enzymes
  5. Electrophoresis 
  6. PCR
  7. Sequencing 
  8. Immunology - cell line maintenance
  9. Human disease diagnostics ELISA
  1. Restriction analysis of DNA
  2. Enzyme Linked Immunosorbant Assay ELISA
  1. 7.01 7.04 DNA Replication & Protein Synthesis

December

  1. Human disease diagnostics cont.
  2. Karyotype
  3. Fluoroscopy
  4. IFA
  5. Bacteria morphology - metabolism
  1. Sterile Technique - Microorganisms
  2. Gram Stain/Identification of Bacteria
  3. Whats in Your Mouth? 
  4. Isolation & Identification of Bacteria
  1. 5.04  Karyotype
  2. 6.01 - 6.03 Bacteria

January

  1. Antibiotic Mechanisms of Action
  2. Internet Investigation of Antibiotics
  3. Antibiotic Presentations
  1. Antibiotic Sensitivity
  2. Internet research  Human Genetic Disease
  3. Multimedia Presentations
  1. 6.01 - 6.03 Bacteria

February

  1. Human genetic disease 
  2. Bioethics 
  3. Plasmid Vectors
  4. Restriction of DNA
  1. Multimedia Presentations
  2. Transformation AMPr E coli MM294
  1. 07.01 - 07.02    Recombinant DNA

March

  1. Oligonucleotide Probe Screening
  2. Northern and Southern hybridization
  3. Eukaryotic and Prokaryotic Gene Regulation 
  4. Homeobox Regulation of Development
  5. Bioethics
  1. Effects of DNA Methylation
  2. Transformation with Plasmid Vector
  3. Mid-log Culture Preparation
  4. Plasmid DNA Purification
  5. Recombination of Antibiotic Genes
  1. 07.01 - 07.02    Recombinant DNA

April

  1. Molecular Biology of Cancer
  2. Human Genome Mapping
  3. Mitochondrial DNA
  4. RFLP VNTR PCR
  5. DNA Diagnosis
  6. Bioethics
  1. Recombinant DNA Transformation
  2. Replica Plating
  3. Recombinant DNA Purification
  4. PCR- 
  5. Mitochondrial DNA
  1. 07.01 - 07.02    Recombinant DNA

May/June

  1. Halorubrum salsolis DNA Sequencing Project
  2. Final Exam
  1. Culture Halophile
  2. Extract Genomic DNA
  3. Digest Halo DNA
  4. Digest pBLUScript Vector
  5. Dephosphorylate Vector
  6. Ligate Halo Fragments
  7. Transform and Clone Halo Inserts
  8. Blue/White Selection of Transformants
  1. Essay Exams
  2. Lab Write-ups
  3. Lab Skill Analysis
  4. Final Exam